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Rooting Hormones May Increase Grafting Success
Research on Apple Grafts using IBA Solutions

Dr. James N. Cummins
N. Y. State Agricultural Experiment Station
Geneva, NY 14456

The following article is reprinted from the Pomona, Spring 1997, Volume 2, number34 XXX. Pomona is the publication of The North American Fruit Explorers (NAFEX, RR3 Box 167, Blomington IL 61704)

Success of a graft union depends on the establishment of a callus bridge between the cut surfaces of scion and stock, and the subsequent establishment of a functioning vascular cylinder connecting scion and stock. Initial callus formation appears to develop about equally on the cut surfaces of both partners, arising not from the cambial layers but from parenchyma cells, mostly in the wood just inside the camblum. Soon after scion and stock calluses have merged, callus cells just below the cambial cells of the scion begin to divide in the same plane as the camblum. Waves of cell division proceed from the top down, suggesting that a regulatory stimulus moves to the cut surface from the growing-shoot tip. Callus parenchyma cells inside the new camblum cylinder re-differentiate into functioning xylem cells. Last of all, the new cambium begins producing phloem cells.

We sought to enhance that regulatory stimulus by applying a solution of a synthetic growth regulator. In the dormant season, we prepared whip-and tongue bench grafts of seven hardy varieties with potential as winter-tolerant frame builders. On half of the grafts of each variety, we applied a 2000 ppm solution of IBA (3-indole butyric acid) [ed note: useHORTUS IBA WATER SOLUBLE SALTS ROOTING SOLUTION @2000 PPM IBA] to the cut surface of the scion before joining stock and scion. After four weeks of callusing in a storage cellar at about 45 to 50F, we planted the grafts in the nursery; during the summer, we provided normal tillage but no irrigation. Trees were harvested in November. We evaluated each set for survival and for tree quality.

With all seven varieties, survival was greater for the IBA-treated grafts than for those not treated; with five of the varieties, all treated grafts survived.

(The following drawing is added to the original article in order to clarify the method)

Whip and Tongue

Survival of Apple Trees
 
I BA
Check
Charlamoff 100% 92%
Delawine 100 83
Delcon 100 57
Kanorkowa 100 82
Wedge 80 35
Ottawa 274 100 78
Ottawa 292 93 69


Apple tree quality of the surviving grafts was as striking as the survival rate, based on grading into salable and non-salable trees:

Saleable Apple Trees

  As % of Survivors As % of Original Grafts
  IBA Check IBA Check
Charlamoff 73% 0% 73% 0%
Delawine 75 10 75 8
Delcon 57 25 57 14
Kanorkowa 33 0 33 0
Wedge 100 67 80 54
Ottawa 274 57 24 57 20
Ottawa 292 71 64 66 44


We have not made microscopic examination of the progress of union establishment and vascularization after IBA treatment, but it seems probable that the IBA is increasing both the formation of callus and the rate at which the new cambium cylinder is differentiated through the callus parenchyma cells.

Using one of the common rooting compounds, such as the 2000 ppm IBA solution reported here, may improve both grafting success and tree quality of bench-grafted trees. Powdered proprietary mixtures could offer advantages over the liquid mixtures and should be tried. The technique is sufficiently promising to merit small-scale trial.

These apple selections were from the Malus catalog of:
National Germplasm Repository for Apple, Grape and Tart Cherry
USDA, ARS Plant Genetic Resources Unit
630 West North Street
Geneva, New York 14456-0462

 

 

Propagation by Air Layering

Air Layer

Air layering involves the wounding the stem of the plant while it is on the stock plant.
Treat the stem with Hortus IBA Water Soluble Salts or hizopon AA #1 or #3 dry powder rooting hormones
• Cover the treated area with peat moss or cocoa fiber.
• Roots form in the covered area.
• After the roots form, cut off the stem from the stock plant and plant in media.

Dry Powder Method

PRODUCTS TO USE
Rhizopon AA #2 or #3 dry powder rooting hormones

HOW TO
• Before using cocoa fibers as the media, make sure the processing salts have been removed.
• Soak the peat moss or cocoa fibers in water until fully absorbed.
• Wound the stem of the cane.
• Use a brush to apply the dry powder Rhizopon #2 or #3 to the wounded area.
• Apply the peat moss or cocoa fibers to the wounded area.
• Cover the moss or cocoa fibers with aluminum foil to protect against drying out.
• Wrap some tape (like electrical tape) at the ends of the foil against the stem to keep the foil from falling off.
• After the roots form, cut off the stem from the stock plant and plant in media.

Solution Method

PRODUCTS TO USE
Hortus IBA Water Soluble Salts

HOW TO
• Make and use a rooting solutions made with Hortus IBA Water Soluble Salts (at 200-500 ppm IBA)
• Before using cocoa fibers as the media, make sure the processing salts have been removed.
• Soak the peat moss or cocoa fibers in the solution until fully absorbed.
• Wound the stem of the cane.
• Apply the peat moss or cocoa fibers to the wounded area.
• Cover the moss or cocoa fibers with aluminum foil to protect against drying out.
• Wrap some tape (like electrical tape) at the ends of the foil against the stem to keep the foil from falling off.
• After the roots form, cut off the stem from the stock plant and plant in media.

 

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